NONPOROUS SPHERICAL POLYMER PARTICLES FOR LUMINESCENT SOLID-PHASE DETECTION REACTORS

Support materials for analytical solid phase detection applications ha ve been prepared. A method for synthesis and functionalization of non- porous particles based on suspension co-polymerization of ethylene dim ethacrylate (EDMA) and 2-hydroxyethyl methacrylate (HEMA) is reported. Attachment of the functionalities 5-amino-8-hydroxyquinoline, and the fluorescent 3-aminofluoranthene, as well as the chemiluminescent isol uminol were accomplished by reaction of the HEMA hydroxyl groups with epichlorhydrin and subsequent attachment of the amino groups to the ox irane group thus introduced. Calcein and Calcein Blue were immobilized through esterification to the HEMA hydroxyl group of the carrier bead . The solid phase reagents were verified qualitatively by colour chang es, fluorescence for the fluorophores, and photon emission for the iso luminol derivative in presence of chlorine dioxide. The particles are intended for analytical solid phase detection and mechanistic studies of luminescence phenomena.