KINETIC-BEHAVIOR OF THE MALONALDEHYDE-THIOBARBITURIC ACID REACTION - KINETIC-FLUOROMETRIC DETERMINATION OF MALONALDEHYDE IN HUMAN SERUM

For the first time a fluorimetric-kinetic method for the determination of malonaldehyde (MLD) is proposed. The fluorescence emission of the thiobarbituric acid (TBA)-MLD reaction product has been monitored at 5 53 nm (excitation wavelength 515 nm), and the instrumental and experim ental variables have been studied. A selective and fast kinetic-fluori metric determination of malonaldehyde is proposed. The sensitivity of the method is very high, the detection limit is 0.30 ng ml(-1), the ap plication range is between 1.1 and 50 ng ml(-1), and the determination time per sample is 500 s. The kinetic-fluorimetric method has been ap plied in the determination of two types of pathological human serum: r heumatic and hyperlipemic serum. In both cases, perchloric acid is use d to precipitate the proteins.