MULTICOLOR WHOLE-MOUNT IN-SITU HYBRIDIZATION TO DROSOPHILA EMBRYOS

Authors
HAUPTMANN G GERSTER T
Citation
G. Hauptmann et T. Gerster, MULTICOLOR WHOLE-MOUNT IN-SITU HYBRIDIZATION TO DROSOPHILA EMBRYOS, Development, genes and evolution, 206(4), 1996, pp. 292-295
Citations number
8
Categorie Soggetti
Developmental Biology","Cell Biology
Journal title
Development, genes and evolution → ACNP
ISSN journal
0949944X
Volume
206
Issue
4
Year of publication
1996
Pages
292 - 295
Database
ISI
SICI code
0949-944X(1996)206:4<292:MWIHTD>2.0.ZU;2-E
Abstract
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluores cein and biotin, respectively, this protocol allows the detection in t hree colours of RNAs derived from three different genes. Hybridized pr obes are detected by consecutive staining with appropriate alkaline ph osphatase conjugates using different chromogenic substrate combination s, and serial removal of the antibody conjugates by low pH washes.