Determination of the density and ionization state of diffuse primordia
l gas in the intergalactic medium (IGM) is an important step towards u
nderstanding the nature of dark matter and the formation of structure
in the Universe. Absorption by neutral hydrogen atoms in the IGM shoul
d produce a large flux deficit in the spectra of high-redshift quasars
at wavelengths below that of the H I Lyman-alpha line (the Gunn-Peter
son effect(1)). But observations of quasars at low spectral resolution
have revealed only relatively small deficits, and at higher spectral
resolution these appear as numerous discrete absorption features (the
Lyman-alpha forest). This implies that intergalactic hydrogen is both
highly ionized and non-uniformly distributed(2). Similar absorption is
expected hom intergalactic helium(3), although its detection is more
challenging and evidence for its existence was obtained only recently(
4,5). Here we report the observation of absorption from singly ionized
helium (He II) in the spectrum of the quasar HS1700 + 64. We measure
a mean flux deficit over the redshift range 2.2 < z < 2.6 that yields
an effective optical depth of 1.00 (+/-0.07). Comparing this result wi
th the H I absorption towards this quasar, we argue that most of the m
easured He II absorption occurs in the lowest-density regions of the I
GM.