A TN1545-LIKE TRANSPOSON CARRIES THE TET(M) GENE IN TETRACYCLINE RESISTANT STRAINS OF BACTEROIDES-UREOLYTICUS AS WELL AS UREAPLASMA-UREALYTICUM BUT NOT NEISSERIA-GONORRHOEAE

The presence of the tet(M) determinant and conjugative transposons rel ated to Tn1545 in urogenital pathogens, Bacteroides ureolyticus (10 st rains), Neisseria gonorrhoeae (37 strains) and Ureaplasma urealyticum (81 strains), was studied by PCR analysis and hybridization assay. All tetracycline-resistant strains that hybridized with a probe specific for tet(M) gave the expected fragment when their DNA's were subject to PCR. The tet(M) gene and int-Tn, the gene encoding the protein requir ed for the movement of Tn1545-like transposons, were always found toge ther in tetracycline resistant strains of B. ureolyticus and U. urealy ticum. In contrast, int-Tn was not detected in tet(M)-mediated tetracy cline-resistant N. gonorrhoeae strains. These data suggest carriage of tet(M) by a Tn1545-like transposon in some tetracycline-resistant B. ureolyticus.