A SIMPLIFIED PROCEDURE FOR THE PURIFICATION OF TRICHOSANTHIN (A TYPE-1 RIBOSOME-INACTIVATING PROTEIN) FROM TRICHOSANTHES-KIRILOWII ROOT TUBERS

Authors
BHATIA N MCDONALD KA JACKMAN AP DANDEKAR AM
Citation
N. Bhatia et al., A SIMPLIFIED PROCEDURE FOR THE PURIFICATION OF TRICHOSANTHIN (A TYPE-1 RIBOSOME-INACTIVATING PROTEIN) FROM TRICHOSANTHES-KIRILOWII ROOT TUBERS, Protein expression and purification, 7(2), 1996, pp. 143-146
Citations number
14
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
Protein expression and purification → ACNP
ISSN journal
10465928
Volume
7
Issue
2
Year of publication
1996
Pages
143 - 146
Database
ISI
SICI code
1046-5928(1996)7:2<143:ASPFTP>2.0.ZU;2-K
Abstract
A one step rapid and simple purification procedure for trichosanthin, a type 1 ribosome inactivating protein, from root tubers of Trichosant hes kirilowii has been developed using cation-exchange perfusion chrom atography. The identity of the protein has been confirmed by its size, immunoreactivity, and sequence information. Yields of 0.16% of electr ophoretically pure trichosanthin hom dried root tuber have been achiev ed with a single 10-min chromatographic step giving the ability to pur ify gram quantities of trichosanthin in 1 day. (C) 1996 Academic Press , Inc.