N-ACETYL-D-GALACTOSAMINE-SPECIFIC LECTINS FROM PHAEOLEPIOTA-AUREA

Citation
H. Kawagishi et al., N-ACETYL-D-GALACTOSAMINE-SPECIFIC LECTINS FROM PHAEOLEPIOTA-AUREA, Phytochemistry, 41(4), 1996, pp. 1013-1016
Citations number
30
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
00319422
Volume
41
Issue
4
Year of publication
1996
Pages
1013 - 1016
Database
ISI
SICI code
0031-9422(1996)41:4<1013:NLFP>2.0.ZU;2-Y
Abstract
Two lectins, PAL-I and PAL-II, were isolated from the mushroom Phaeole piota aurea by affinity chromatography on acid-treated Sepharose CL-4B followed by reverse-phase FPLC on ProRPC. Both of the lectins were te tramers of 16 kDa subunits. The lectins had little agglutination activ ity against native erythrocytes but Pronase treatment of erythrocytes increased the sensitivity to agglutination by the lectins. Both lectin s exhibited slight preferences for type A compared with type B and O e rythrocytes. In haemagglutination inhibition assays, N-acetylgalactosa mine and both anomers of methyl N-acetylgalactosaminide were the best inhibitors.