FLUOROMETRIC ASSAY FOR HORSERADISH-PEROXIDASE IN ORGANIC MEDIA

A fluorometric method for assaying the activity of horseradish peroxid ase (HRP) in organic media has been developed. This method is designed on the basis of the disparity in the spectral properties of substrate s and corresponding resultant polymers. It monitors the fluorescence q uenching of substrate during enzymatic catalysis, and works efficientl y in a number of organic media (such as dioxane-water mixture, acetone -water mixture, and alcohol-water mixture, and so forth) toward many s ubstrates. This assay is simpler, more rapid, and more convenient comp ared with the commonly used HPLC method. It is qualitatively reproduci ble and can also be used for quantitative calculation of the substrate conversion.