POSTELECTROPHORESIS CAPILLARY SCANNING METHOD FOR DNA-SEQUENCING

A postelectrophoresis capillary scanning method for increasing the thr oughput of DNA sequencing has been developed, The method features a sp atially and temporally separated arrangement between capillary gel ele ctrophoresis separation of DNA sequencing fragments and the visualizat ion of the separation pattern, Fluorescently labeled DNA sequencing fr agments of pBluescript SK(-) were produced in enzymatic chain-terminat ion reactions and separated by capillary gel electrophoresis using a 1 10 cm fused silica capillary with a transparent polymer coating. A par tial sequence from the DNA was then determined by repeated scanning of the capillary at a rate of 8.39 cm/s, with changing excitation wavele ngths and spectral filter sets.