S. Yu et al., IMMUNOHISTOCHEMICAL LOCALIZATION OF GLUCOSE TRANSPORTERS (GLUT1 AND GLUT3) IN THE RAT HYPOTHALAMUS, Obesity research, 3, 1995, pp. 753-760
Immunohistochemical localization of the glucose transporters was studi
ed in the rat hypothalamus by using a specific rabbit antiserum raised
against either the isoform 1 (GLUT1) or the isoform 3 (GLUT3). Immuno
reactive staining for GLUT1 was found in glia cells and capillaries, w
hereas positive staining for GLUT3 occurred mainly in neurons and part
ly in ependymal cells, Double immunostaining indicated that a small po
pulation of GLUT1-positive cells were reactive for glial fibrillary ac
idic protein, a marker for astrocytes, Another doubly stained section
showed that GLUT1-positive glia cells were never stained with an OX42
antibody, a marker for microglia cells, Neurons staining positively fo
r GLUT3, often large in cell size, were confined mainly in the lateral
hypothalamic area and partly in the dorsomedial and periventricular h
ypothalamic nuclei. Possible significance of these two glucose transpo
rters in the hypothalamus is briefly discussed.