Na. Babenko et Ns. Kavok, EFFECTS OF THYROID-HORMONES, PHORBOL ESTERS, AND SPHINGOSINE ON THE INCORPORATION OF LINOLEIC-ACID INTO LIPIDS IN THE LIVER OF WHITE-RATS, Biochemistry, 60(12), 1995, pp. 1545-1550
Effects of thyroxine, phorbol esters, and sphingosine on the incorpora
tion of [C-14]linoleic acid into endogenous phospholipids, triacylglyc
erols, and cholesterol esters in the liver of white rats were studied.
Phorbol 12-myristate 13-acetate and thyroxine activated the incorpora
tion of [C-14]linoleic acid into endogenous phospholipids and triacylg
lycerols in liver cells. However, phorbol 12-myristate 13-acetate 4-O-
methyl ether had no effect, and methimazole and sphingosine suppressed
the incorporation of fatty acids into glycerolipids in liver. The lat
ter abolished the activating effect of thyroxine on this process. Sphi
ngosine, thyroxine, and phorbol 12-myristate 13-acetate 4-O-methyl eth
er did not affect the synthesis of cholesterol esters, but phorbol 12-
myristate 13-acetate increased this process twofold in liver cells.