THE ROLE OF SALIVA AND SERUM IN CANDIDA-ALBICANS BIOFILM FORMATION ONDENTURE ACRYLIC SURFACES

Citation
H. Nikawa et al., THE ROLE OF SALIVA AND SERUM IN CANDIDA-ALBICANS BIOFILM FORMATION ONDENTURE ACRYLIC SURFACES, Microbial ecology in health and disease, 9(1), 1996, pp. 35-48
Citations number
39
Categorie Soggetti
Microbiology,Ecology
ISSN journal
0891060X
Volume
9
Issue
1
Year of publication
1996
Pages
35 - 48
Database
ISI
SICI code
0891-060X(1996)9:1<35:TROSAS>2.0.ZU;2-J
Abstract
The long term effect of either a salivary or a serum pellicle on Candi da albicans biofilm formation on denture acrylic surfaces was investig ated both by quantifying the ATP (adenosine triphosphate) content of t he resultant biofilms and by scanning electron microscopy. When the bi ofilm formation on saliva-coated acrylic strips was examined, the yeas ts initially colonised this surface at a slower rate than the controls although with increasing incubation time, at 72 h, the ATP content wa s almost ten-fold higher than the protein-free control strips. Ultrast ructural studies revealed this to be due to cell aggregation and hypha l emergence, phenomena not observed in the controls. As compared with the control strips, biofilm activity of the serum-coated strips was al most 100-fold greater within 48 h incubation, and scanning electron mi croscopy revealed multilayer blastospore-blastospore co-adhesion, germ tube, hyphal and pseudohyphal emergence and blastospore-hyphal coadhe rence. Further immunocytochemical observation revealed that concanaval in-A binding material and fibronectin were involved in biofilm formati on on both saliva and serum coated specimens and, in addition, mannan- binding protein and protein-A binding material also contributed to the biofilm formation on serum coated specimens.