DNA-SEQUENCE DATA IN PHYLOGENY RECONSTRUCTION - AN OVERVIEW OF TECHNIQUES AND ANALYSES

This review provides descriptions of the molecular biological techniqu es of PCR and DNA sequencing and the analysis of DNA sequence variabil ity for discerning phylogenetic relationships. There is a brief discus sion of three general categories of phylogenetic analysis, including t hose based on pairwise distances, parsimony, and maximum likelihood. L astly, techniques for assessing the robustness of phylogenetic hypothe ses and for finding multiple islands of equally-parsimonious trees are reviewed.