CORRELATED EXPRESSION OF MATRIX METALLOPROTEINASES AND ETS FAMILY TRANSCRIPTION FACTOR E1A-F IN INVASIVE ORAL SQUAMOUS-CELL-CARCINOMA-DERIVED CELL-LINES
M. Shindoh et al., CORRELATED EXPRESSION OF MATRIX METALLOPROTEINASES AND ETS FAMILY TRANSCRIPTION FACTOR E1A-F IN INVASIVE ORAL SQUAMOUS-CELL-CARCINOMA-DERIVED CELL-LINES, The American journal of pathology, 148(3), 1996, pp. 693-700
Matrix metalloproteinases (MMPs) have been implicated irt invasion and
metastasis of tumor cells. Transcription regulatory regions of MMP ge
nes often contain binding sites for ets transcription factors. We rece
ntly isolated a cDNA encoding human E1A-F, a member of the ets oncogen
e family, and showed that E1A-F; can upregulate MMP genes by CAT assay
. We attempted to investigate the relationship between E1A-F mRNA expr
ession and MMP protein expression in four different types of oral squa
mous-cell-carcinoma-derived cell lines (HSC 3, SAS, KB, and Ca 9.22).
HSC 3 and SAS are highly invasive cell lines when they are injected in
the tongue of nude mice Raft culture of HSC 3 and SRS revealed the sa
me characteristics as seen in tumors implanted in viva Both type I col
lagenase (MMP-1) and 92-kd type IV collagenase (MMP-9) were detected i
n cultured HSC 3 and SAS cells. E1A-F mRNA was demonstrated to be high
ly expressed in HSC 3 and SAS by Northern blotting, and in situ hybrid
ization confirmed E1A-F mRNA expression at the invasion front of tumor
cells seeded on collagen gel. On the other hand, RE and Ca 9.22 have
little potential for invasion, and MMP-1 and MMP-9 protein and E1A-F m
RNA could not be detected. These results suggest that the ets-related
E1A-F participates in the regulation of invasion-associated MMP genes
and is involved ia presenting invasive activity in tumor cells of oral
squamous cell carcinoma.