RAPID, SMALL-SCALE PURIFICATION OF RICE HOJA BLANCA AND ECHINOCHLOA HOJA BLANCA TENUIVIRUS RIBONUCLEOPROTEIN

Highly purified tenuivirus ribonucleoprotein was obtained from small a mounts of leaf tissue by sedimenting the ribonucleoprotein particles f rom debris-free plant extract into a 30% sucrose cushion, in 1.5-ml mi crofuge tubes. Using this protocol, significant size differences were discovered in the double-stranded forms of the viral genomic RNAs of r ice hoja blanca tenuivirus and a tenuivirus isolated from Echinochloa colonum, a common weed of rice cultivation.