SEROLOGICAL DIAGNOSIS OF AVIAN SALMONELLO SIS - DEVELOPMENT OF AN ELISA TEST USING ANTIGENS ADSORBED WITH ANTI-ESCHERICHIA-COLI SERA

A serological ELISA test for diagnosis of avian salmonellosis infectio ns with Salmonella typhimurium or enteritidis has been established. Pl ates were coated half with a negative antigen and half with a positive antigen. Both negative and positive antigens were adsorbed with a mon ovalent agglutinant anti Escherichia coli serum prior to being distrib uted into wells of the plates. Sera from SPF birds and from SPF birds vaccinated and/or inoculated with numerous viruses or bacteria, or ser a from conventional birds bacteriologically free of any salmonellosis were tested and the percentage of false positive reactions was inferio r than 1 %. In groups of birds naturally infected or experimentally in oculated with Salmonella enteritidis or typhimurium t e percentages of positive individuals were ranged from 15 to more than 90 % according to the doses and routes of inoculation and the delay between contamina tion and sampling.