EFFICACY OF VARIOUS NON-OILY ADJUVANTS IN IMMUNIZATION AGAINST THE AUJESZKYS-DISEASE (PSEUDORABIES) VIRUS

Standard oil and various non-oily adjuvants were compared for use in i mmunization against the Aujeszky's disease (pseudorabies) virus, both in mice and swine, and using either inactivated virions or purified gl ycoproteins as antigen. Mineral oil, sodium alginate, aluminium hydrox ide, and saponin were assayed in mice as adjuvants for inactivated vir ions, saponin being the most efficient. The addition of Mab anti-CD3 d id not improve either immune response or protection achieved in mice u sing viral particles with oil or sodium alginate. When purified glycop roteins were used as antigens, the use of ISCOM greatly enhanced speci fic T-cell responses and protection of mice. The incorporation of Mab anti-CD3 into ISCOM conferred 100 % protection of mice. Surprisingly, when an ISCOM containing glycoproteins was assayed in swine in a singl e-dose trial, no improvement on the protection conferred by the oily a djuvant was observed.