HUMAN-ANTIBODY FRAGMENTS SPECIFIC FOR HUMAN BLOOD-GROUPS ANTIGENS FROM A PHAGE DISPLAY LIBRARY

We have isolated human antibody fragments with binding specificities a gainst the blood group antigens of the ABO and I blood group systems ( B and HI), of the Rh system (D and E) and of the Kell system (Kp(b)), by selecting a phage-antibody library on human red cells. The fragment s, expressed in bacteria, were antigen-specific and effective in assay s including agglutination and immunohistochemistry. Selection of phage -antibody libraries using intact cells seems to offer a promising alte rnative to hybridoma technology for the production of antibodies again st cell surface molecules.