STRUCTURAL MODIFICATION OF A PERIODIC POLYPEPTIDE THROUGH BIOSYNTHETIC REPLACEMENT OF PROLINE WITH AZETIDINE-2-CARBOXYLIC ACID

Repetitive polypeptides comprising 16 repeats of the sequence -(AlaGly )(3)ProGluGly- (1a) have been prepared from Escherichia coli as overex pressed recombinant proteins. Partial in vivo replacement of the proli ne (Pro) residues in sequence 1a with L-azetidine-2-carboxylic acid (A te) was achieved by expression of the target protein in medium contain ing Ate and lacking Pro. NMR and amino acid analysis for residual prol ine in the polymer indicated 25-40% replacement of Pro by Ate. While p olymers of la form conformationally disordered solids, incorporation o f Aze allows this material to adopt a beta-sheet structure in the soli d state.