Jb. Gelderd et al., THE EFFECTS OF INTERLEUKIN-1 RECEPTOR ANTAGONIST PROTEIN (IRAP) INFUSION FOLLOWING SPINAL-CORD TRANSECTION IN RATS, Molecular and chemical neuropathology, 27(2), 1996, pp. 167-183
A laminectomy was performed at the T5-T6 vertebral level in adult, mal
e, Sprague-Dawley rats and the spinal cord transected with a scalpel.
A group of sham animals was subjected to the same surgery without the
transection step. A group of unhandled control rats was also included.
A subgroup of transected animals received a subcutaneous osmotic mini
pump that dispensed IL-1 receptor antagonist protein (IRAP) at the tra
nsection site for 7 consecutive days. Another transected subgroup rece
ived a minipump that infused the vehicle only. IRAP-treated rats displ
ayed a significant reduction in body temperature (p < 0.05) compared w
ith vehicle-treated rats. The IRAP-treated rats were also less active
when assessed for locomotor behavior using an HVS computerized trackin
g system (p < 0.01). IRAP treatment had no effect on serum corticoster
one, beta-endorphin levels, Con A, PHA, or LPS-induced splenocyte mito
genesis when compared with vehicle-treated animals. However, half of t
he IRAP-treated animals exhibited a substantive reduction in the numbe
r of reactive astrocytes near the transection site, suggesting a possi
ble effect of IRAP on astrocyte activation.