COMPUTER OPTIMIZATION FOR RP-HPLC SEPARATION OF SOME NUCLEOSIDES

Authors
DZIDO TH SORY A
Citation
Th. Dzido et A. Sory, COMPUTER OPTIMIZATION FOR RP-HPLC SEPARATION OF SOME NUCLEOSIDES, Chemia Analityczna, 41(1), 1996, pp. 113-120
Citations number
9
Categorie Soggetti
Chemistry Analytical
Journal title
Chemia Analityczna → ACNP
ISSN journal
00092223
Volume
41
Issue
1
Year of publication
1996
Pages
113 - 120
Database
ISI
SICI code
0009-2223(1996)41:1<113:COFRSO>2.0.ZU;2-Z
Abstract
The DrylabG software (LC Resources, Lafayette, CA, USA) was applied fo r optimization of HPLC resolution of some nucleosides in the reversed- phase systems. Two preliminary runs based on linear gradient range of acetonitrile (ACN) from 0% (pure buffer) to 20% and of methanol (MeOH) from 0% to 50% are shown to be satisfactory for optimization of the r esolution. A good agreement between simulated and experimental chromat ograms was observed.