CLONING AND SEQUENCING OF 2 GENES FROM STAPHYLOCOCCUS-CARNOSUS CODINGFOR GLUCOSE SPECIFIC PTS AND THEIR EXPRESSION IN ESCHERICHIA-COLI K-12

Citation
I. Christiansen et W. Hengstenberg, CLONING AND SEQUENCING OF 2 GENES FROM STAPHYLOCOCCUS-CARNOSUS CODINGFOR GLUCOSE SPECIFIC PTS AND THEIR EXPRESSION IN ESCHERICHIA-COLI K-12, MGG. Molecular & general genetics, 250(3), 1996, pp. 375-379
Citations number
16
Categorie Soggetti
Genetics & Heredity",Biology
ISSN journal
00268925
Volume
250
Issue
3
Year of publication
1996
Pages
375 - 379
Database
ISI
SICI code
0026-8925(1996)250:3<375:CASO2G>2.0.ZU;2-D
Abstract
Phosphoenolpyruvate (PEP)-dependent phosphorylation experiments have i ndicated that the gram-positive bacterium Staphylococcus carnosus poss esses an EIICBA fusion protein specific for glucose. Here we report th e cloning of a 7 kb genomic DNA fragment containing two genes, glcA an d glcB, coding for the glucose-specific PTS transporters EII(Glc)1 and EII(Glc)2 which are 69% identical. The translation products derived f rom the nucleotide sequence consist of 675 and 692 amino acid residues and have calculated molecular weights of 73 025 and 75 256, respectiv ely. Both genes can be stably maintained in Escherichia coli cells and restore the ability to ferment glucose to ptsG deletion mutants of E. coli. This demonstrates the ability of the PTS proteins HPr and/or EI IA(Glc) of a gram-negative organism (E. coli) to phosphorylate an EIIC BA(Glc) from a gram-positive organism (S. carnosus).