XANTHOMONAS-CAMPESTRIS AS A HOST FOR THE PRODUCTION OF RECOMBINANT PSEUDOMONAS-AERUGINOSA LIPASE

Recombinant plasmid pBP13, which expresses the alkaline lipase from Ps eudomonas aeruginosa IGB83 under the tac promoter was transferred to X anthomonas campestris pv campestris IBT148, Different fermentation con ditions were tested for lipase productivity by strain IBT148 carrying plasmid pBP13, and a fermentation process was established in an instru mented bioreactor, where lipase production was increased more than 12- fold with respect to the initial culture conditions in shake flasks. X anthan gum stabilized the activity of the alkaline lipase.