N. Aitabdelkader et al., ISOLATION AND PROPERTIES OF MUTANTS OF ZYMOMONAS-MOBILIS DEFICIENT INSUGAR ASSIMILATION, Applied and environmental microbiology, 62(3), 1996, pp. 1096-1098
An enrichment method using D-cycloserine was designed for the isolatio
n of spontaneous mutants of Zymomonas mobilis deficient in glucose or
fructose utilization. The mutants could easily be isolated since they
represented 80 to 90% of the population after two and three enrichment
cycles. Glucokinase and fructokinase activities in the mutants were a
ffected.