XANTHONE INDUCTION OF NOD GENE-EXPRESSION IN BRADYRHIZOBIUM-JAPONICUM

Citation
Jpy. Yuen et al., XANTHONE INDUCTION OF NOD GENE-EXPRESSION IN BRADYRHIZOBIUM-JAPONICUM, Symbiosis, 19(2-3), 1995, pp. 131-140
Citations number
25
Categorie Soggetti
Microbiology
Journal title
ISSN journal
03345114
Volume
19
Issue
2-3
Year of publication
1995
Pages
131 - 140
Database
ISI
SICI code
0334-5114(1995)19:2-3<131:XIONGI>2.0.ZU;2-O
Abstract
Forty aromatic compounds were tested for their ability to induce nod g ene expression in Bradyrhizobium japonicum. Of these, genistein, bioch anin A, and formononetin have previously been shown to be inducers of both nodD(1)-lacZ and nodY-lacZ expression. In addition, two xanthones -1,3,7-trihydroxyxanthone and 1,6-dihpdroxy-2,8-dimethoxyxanthone, wh ich were isolated from Haploclathra species - were found to be inducer s of nod gene expression. Indeed, 1,6-dihydroxy-2,8-dimethoxyxanthione was as effective as genistein in inducing nodD(1)- and nodY-lacZ expr ession. These xanthone molecules were capable of inducing nod gene exp ression in B. japonicum mutant strain Delta 1267, lacking the nodD(1), nodD(2), and nolA genes. These results suggest that the xanthone indu cers are capable of activating the NodV/W two-component regulatory sys tem known to be essential for nod gene expression in B. japonicum.