DETERMINATION OF ALCOHOLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION AFTER PRECOLUMN DERIVATIZATION WITH 2-(4-CARBOXYPHENYL)-6-METHOXYBENZOFURAN
Ai. Hajyehia et Lz. Benet, DETERMINATION OF ALCOHOLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUOROMETRIC DETECTION AFTER PRECOLUMN DERIVATIZATION WITH 2-(4-CARBOXYPHENYL)-6-METHOXYBENZOFURAN, Journal of chromatography, 724(1-2), 1996, pp. 107-115
Citations number
22
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A simple procedure is described for the derivatization of primary shor
t- and long-chain alcohols and the high-performance liquid chromatogra
phic separation and determination of the resulting derivatives. The al
cohols were derivatized to their 2-(4-carboxyphenyl)-6-methoxybenzofur
an esters with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochlo
ride as the dehydrating agent. The coupling (esterification) reaction
proceeded rapidly and smoothly in the presence of a base catalyst. An
aliquot of the reaction mixture was analyzed either directly or after
further purification using solid or solvent extraction procedures. The
resulting esters of 30 alcohols were separated on a reversed-phase co
lumn (Ultrasphere C-8) with gradient elution and detected fluorimetric
ally (excitation at 315 nm, emission at 390 nm). The limits of detecti
on (signal-to-noise ratio = 3) for the derivatized alcohols were in th
e range 0.1-0.5 pg per injection.