DETERMINATION OF CARBENDAZIM IN WATER BY HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY ONLINE WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH DIODE-ARRAY OR MASS-SPECTROMETRIC DETECTION
Dh. Thomas et al., DETERMINATION OF CARBENDAZIM IN WATER BY HIGH-PERFORMANCE IMMUNOAFFINITY CHROMATOGRAPHY ONLINE WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH DIODE-ARRAY OR MASS-SPECTROMETRIC DETECTION, Journal of chromatography, 724(1-2), 1996, pp. 207-217
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
An automated method for the determination of carbendazim in water that
combines high-performance immunoaffinity chromatography (HPIAC), high
-performance liquid chromatography (HPLC) in the reversed-phase mode,
and detection by either UV-Vis diode array detector (DAD) spectroscopy
or mass spectrometry (MS) is presented here. This method allows for t
he on-line extraction, preconcentration, and positive confirmation of
carbendazim with a throughput of one sample analyzed every 10 min. The
method requires minimal manual sample pretreatment, yet it is free fr
om coextracted interferences that often occur in solid-phase extractio
ns based on nonspecific sorbents. The linear range of the calibration
curve for 200-mu l injections of carbendazim is 0.025 to 100 mu g/l fo
r HPIAC-HPLC-MS and 0.075 to 100 mu g/l for HPIAC-HPLC-DAD. Because th
e calibration curve is mass-dependent, lower detection limits could be
achieved by applying larger sample volumes to the HPIAC column. The w
ithin-day precision is +/-4.5% for HPIAC-HPLC-MS and +/-16% for HPIAC-
HPLC-DAD for samples containing 0.1 mu g/l carbendazim. The results fr
om this method correlate well with results from an ELISA.