INTRODUCTION OF MUTATOR PHAGE D3112 OF PS EUDOMONAS-AERUGINOSA INTO ALCALIGENES-EUTROPHUS VAR METALLOTOLLERANS (STRAIN CH34)

It is demonstrated that the intact genome of a D3112 transposable phag e (TP) of Pseudomonas aeruginosa, integrated into a recombinant plasmi d RP4::D3112, can be transferred by means of conjugation from P. putid a PpG1 (RP4npt::D3112) donor cells into Alcaligenes eutrophus var. met allotollerans cells. P. aeruginosa strains are unacceptable as donors because they have a bactericidal effect on A. eutrophus. RP4npt::D3112 plasmid is stably inherited by A. eutrophus with D3112 being expresse d and successfully reproduced. However, TP loses the induction ability after UV irradiation or mitomycin C treatment. It is suggested that D 3112 TP and its miniderivatives could be used in manipulations with A. eutrophus var. metallotolerans.