PROGRESSIVE CELLULAR DEHYDRATION AND PROTEOLYSIS IN CRITICALLY ILL PATIENTS

Background According to a recent hypothesis, the profound loss of body protein that occurs in critically ill patients is triggered and maint ained by cell shrinkage secondary to cellular dehydration. We tested t his hypothesis by studying sequential changes in intracellular water, total body protein, total body potassium, and intracellular potassium in patients receiving intensive care for blunt trauma or sepsis. Metho ds Nine patients with multiple blunt trauma and 11 with severe sepsis were studied in an intensive care unit for 21 days. Intracellular wate r was measured in two ways--by subtraction of extracellular water (bro mide dilution) from total body water (tritium dilution), and by bioimp edance spectroscopy. Total body protein was measured by whole-body neu tron activation analysis and total body potassium by whole-body counti ng. Findings Over the study period intracellular water decreased by 15 -20%, total body protein by 15%, and total body potassium by about 20% . Intracellular potassium concentration did not change, and was simila r to that in healthy adult volunteers. In the trauma patients, sequent ial measurements of the ratio of potassium to protein In lost tissue i ndicated that cells were losing water in quantities greater than would be expected from protein losses. Interpretation The loss of protein a nd potassium from body stores in major trauma or sepsis is accompanied by progressive cellular dehydration. This insight opens up new therap eutic options for limiting the loss of body protein in critically ill patients.