NONRADIOACTIVE MONITORING OF ORGANIC AND INORGANIC SOLUTE TRANSPORT INTO SINGLE XENOPUS OOCYTES BY CAPILLARY ZONE ELECTROPHORESIS

Transport of organic and inorganic solutes into and out of cells requi res specialized transport proteins. Given a sufficiently sensitive ana lytical method for measuring cellular solute concentrations, it should be possible to monitor solute transport across the plasma membrane al the level of single cells. We report a capillary zone electrophoresis approach that is generally applicable to monitor solute transport int o Xenopus laevis oocytes, requires only nanoliters of sample, and invo lves no radioactive materials. The sensitivity of capillary electropho resis with UV detection is typically on the order of 10(-5)-10(-6) M, resulting in the mass detection limits in the low femtomole range. We show that capillary zone electrophoresis serves as a simple technique to measure solute transport into oocytes. Studies of the mammalian oli gopeptide transporter PepT1 and the Na+- and K+-coupled epithelial and neuronal glutamate transporter EAAC1 expressed in oocytes demonstrate that transport of the dipeptide Trp-Gly via PepT1 and transport of Na + and K+ via EAAC1 across the oocyte plasma membrane can be monitored by measuring intracellular tryptophan absorption and by indirect UV de tection of inorganic ions, respectively. The CZE method allowed the si multaneous detection of changes of intracellular Na+ and K+ concentrat ions in response to EAAC1-mediated Na+ cotransport and K+ countertrans port. This is the first report of a capillary zone electrophoresis-bas ed quantitative analysis of intracellular components of a single cell in response to transport activity.