ESTABLISHMENT OF A CONTINUOUS CELL-LINE FROM A HUMAN CARCINOID OF THESMALL-INTESTINE (KRJ-I) - CHARACTERIZATION AND EFFECTS OF 5-AZACYTIDINE ON PROLIFERATION

Authors
PFRAGNER R WIRNSBERGER G NIEDERLE B BEHMEL A RINNER I MANDL A WAWRINA F LUO JS ADAMIKER D HOGER H INGOLIC E SCHAUENSTEIN K
Citation
R. Pfragner et al., ESTABLISHMENT OF A CONTINUOUS CELL-LINE FROM A HUMAN CARCINOID OF THESMALL-INTESTINE (KRJ-I) - CHARACTERIZATION AND EFFECTS OF 5-AZACYTIDINE ON PROLIFERATION, International journal of oncology, 8(3), 1996, pp. 513-520
Citations number
52
Categorie Soggetti
Oncology
Journal title
International journal of oncology → ACNP
ISSN journal
10196439
Volume
8
Issue
3
Year of publication
1996
Pages
513 - 520
Database
ISI
SICI code
1019-6439(1996)8:3<513:EOACCF>2.0.ZU;2-7
Abstract
A new continuous cell line from a human malignant carcinoid of the sma ll intestine (KRJ-I) was established. The cells showed morphological a nd immunocytochemical features of the tumor of origin and expressed es trogen receptors. The cells are growing as a suspension, forming multi cellular aggregates and spheroids. Electron microscopy confirmed the p resence of neuroendocrine granules. Dose-dependent growth inhibition w as observed after incubation with 5-azacytidine. Cytogenetic analyses of the tumor of origin, the cell line KRJ-I and a liver metastasis KRJ -II revealed clonal tetraploidy and clonal loss of the Y-chromosome an d chromosome 19.