COMPARISON OF THE MESSENGER-RNA EXPRESSION OF FACTORS RELATED TO DRUG-RESISTANCE IN LUNG-TUMORS AND ADJACENT NORMAL TISSUE

Drug resistance related proteins P-glycoprotein (P170), multidrug resi stance associated protein (MRP), glutathione S-transferase-pi (GST-pi) , glutathione peroxidase (GPX), topoisomerase II (Topo II), thymidylat e synthase (TS), O-6-methylguanine-DNA-methyltransferase (MGMT), the h eat shock proteins HSP27 and HSP70 and the protooncogenes Fos, Jun and EGFR were investigated in human lung carcinomas and matched normal ti ssues. We found that the mRNA expression of Topo II and TS were elevat ed in tumor tissue versus corresponding normal tissue. Additionally To po II and TS correlated with the proliferating activity determined by expression of histone 3. P170, MRP, HSP70 and also EGFR mRNA were elev ated in some tumor probes, but not GST-pi, GPX, MGMT and HSP27 mRNA. A dditionally, we determined various values of Fos and Jun mRNA expressi on but there was no uniform pattern. The finding that some proteins we re abnormally expressed in lung tumors compared to the adjacent normal tissue is an important finding for further investigations on the deve lopment of individualized chemotherapy but more samples should be exam ined to extend these observations.