EFFECT OF DEOXYMANNOJIRIMYCIN AND BREFELDIN-A ON YERSINIA-PSEUDOTUBERCULOSIS INVASIN-EUKARYOTIC CELL-INTERACTION

The cell surface receptors of the Yersinia pseudotuberculosis invasin protein are the alpha(3-6)beta(1) integrins. Invasin and the extracell ular matrix protein fibronectin bind to the same or closely located si tes on alpha(5) beta(1) integrin, although invasin bind with a much gr eater affinity. Invasin-integrin interaction promotes bacterial penetr ation into eukaryotic cells. Binding of fibronectin to its integrin re ceptor seems to be dependent on terminal oligosaccharides processing. In this paper, we have examined the effect of 1-deoxymannojirimycin (d MNJ), an inhibitor of Golgi alpha-mannosidases involved in processing of N-glycan precursors, and of Brefeldin A (BFA), a natural product of fungi which has profound effects on the structure and function of the Golgi apparatus, on invasin-cell interaction. We found that unlike fi bronectin, the interaction of invasin with cells was resistance to dMN J. However, preincubating cells with BFA caused a dose dependent inhib ition of invasin-mediated cell entry, while cell invasion by Salmonell a typhimurium was not affected. (C) 1995 Academic Press Limited.