ISOLATION OF STRONG PROMOTERS FROM CLAVIBACTER-XYLI SUBSP CYNODONTIS USING A PROMOTER PROBE PLASMID

Authors
HAAPALAINEN M KARP M METZLER MC
Citation
M. Haapalainen et al., ISOLATION OF STRONG PROMOTERS FROM CLAVIBACTER-XYLI SUBSP CYNODONTIS USING A PROMOTER PROBE PLASMID, Biochimica et biophysica acta, N. Gene structure and expression, 1305(3), 1996, pp. 130-134
Citations number
22
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1305
Issue
3
Year of publication
1996
Pages
130 - 134
Database
ISI
SICI code
0167-4781(1996)1305:3<130:IOSPFC>2.0.ZU;2-1
Abstract
To isolate promoters from Clavibacter xyli subsp. cynodontis (C. xyli subsp. cynodontis), we constructed a new promoter probe plasmid and ma de a C. xyli subsp. cynodontis promoter probe library. Two promoters g ave over 2500-times stronger expression than the parental plasmid. The promoters were sequenced and compared to other bacterial promoters. T hese C. xyli subsp. cynodontis promoter regions are GC-rich and do not resemble E. roll promoters, but do resemble a few individual promoter s found in streptomycetes.