PARTIAL-PURIFICATION AND CHARACTERIZATION OF 2 TYPES OF HOMOLOGOUS DNA PAIRING ACTIVITY FROM RAT TESTIS NUCLEI

Authors
NAMSARAEV EA LANZOV VA AKHMEDOV AT
Citation
Ea. Namsaraev et al., PARTIAL-PURIFICATION AND CHARACTERIZATION OF 2 TYPES OF HOMOLOGOUS DNA PAIRING ACTIVITY FROM RAT TESTIS NUCLEI, Biochimica et biophysica acta, N. Gene structure and expression, 1305(3), 1996, pp. 172-180
Citations number
51
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1305
Issue
3
Year of publication
1996
Pages
172 - 180
Database
ISI
SICI code
0167-4781(1996)1305:3<172:PACO2T>2.0.ZU;2-3
Abstract
We describe the partial purification and characterization of two diffe rent types of homologous DNA pairing activity from rat testis nuclear extracts. The activities are separated from each other by single-stran ded DNA-cellulose affinity chromatography. One activity requires singl e-stranded DNA ends and promotes the homologous pairing of single-stra nded DNA fragments with double-stranded circular DNA and has an appare nt molecular mass of 100 kDa as determined by gel filtration chromatog raphy. This pairing activity does not require the addition of exogenou s ATP and is strongly Mg2+-dependent. The second pairing activity prom otes strand-transfer between single-stranded circular DNA and homologo us double-stranded DNA fragments and has an apparent molecular mass of 30 kDa as determined by gel filtration chromatography. This pairing a ctivity also does not require ATP but, in contrast to the former, is M g2+-independent.