SEPARATION OF ENANTIOMERS USING VANCOMYCIN IN A COUNTERCURRENT PROCESS BY SUPPRESSION OF ELECTROOSMOSIS

Excellent separations were achieved using a coated column to suppress electroosmotic now and employing a countercurrent process between chir al selector and racemic solute. Using the macrocyclic antibiotic vanco mycin as a chiral selector in capillary electrophoresis the resolution of nonsteroidal antiinflammatories and dansyl amino acids was achieve d. Improvement in sensitivity due to the elimination of background abs orbance and increased efficiency due to the removal of wall adsorption effects are both achieved using this technique. (C) 1996 Wiley-Liss, Inc.