C. Bai et al., A HIGHLY SENSITIVE HPLC METHOD TO DISCRIMINATE ENANTIOMERIC AMINO DEOXY SUGARS BASED ON THE DERIVATIZATION WITH (S)-(-TBMB CARBOXYLIC-ACID()), Journal of carbohydrate chemistry, 15(2), 1996, pp. 217-230
A picomole determination of the absolute configurations of amino deoxy
sugars was demonstrated through the derivatization at the anomeric ca
rbon with a fluorescent chiral reagent, (S)-(+)-TBMB carboxylic acid,
followed by the HPLC separations of the derived diastereomeric isomers
. Anew chemical procedure was developed to convert amino deoxy sugars
into per-NH,O-acetylated glycosyl chlorides prior to derivatization wi
th the reagent.