Mp. Singh et al., EFFECT OF CRYOPROTECTANTS ON RELEASE OF VARIOUS ENZYMES FROM BUCK SPERMATOZOA DURING FREEZING, Theriogenology, 45(2), 1996, pp. 405-416
Semen samples from 12 bucks were extended with 10 different extenders
containing glycerol, DMSO, glycerol + DMSO, and glycerol + lactose in
varying concentrations as cryoprotective agents. The activities of acr
osin, hyaluronidase, alkaline phosphatase (AKP), aspartate aminotransf
erase (AST), alanine amino transferase (ALT) and lactic dehydrogenase
(LDH) were assayed in equilibrated (Prefreeze) and frozen-thawed (Post
freeze) semen samples. Significantly (P < 0.01) higher intracellular a
ctivity of acrosin was recorded in semen samples extended with lactose
than with the other extenders, with the maximum being with Tris yolk
glycerol lactose (TYGL(180)). Effects of extenders on acrosin activity
were significant (P < 0.01) at both of the pre-and postfreeze stages.
However, extracellular activities of hyaluronidase, alkaline phosphat
ase, transaminases (AST and ALT), and lactic dehydrogenase were signif
icantly higher in extenders containing DMSO then lactose. Leakage of t
hese enzymes was found to increase from the prefreeze to the postfreez
e stage.