DETECTION OF THE A-ALLELE AND B-ALLELE ON KAPPA-CASEIN LOCUS IN THE BOHEMIAN RED STRAIN

The frequencies of the A and B alleles of kappa-casein in the 25 indiv iduals of Bohemian Red strain were detected by the polymerase chain re action (PCR) and by the restriction fragments length polymorphism (RFL P). This old strain belongs to the endangered gene reserves. The DNA w as isolated from the blood. For PCR the primers JK 501 (5'-ATCATITATGG CCATTCCACCAAAG-3') and JK 302 (5'-GCCCATTTCGCCTTCTCTGTAACAGA-3') were used, the PCR product was split by the restriction enzyme HindIII. The AA genotype was found in 6 animals, genotype AB in 14 and BE in 5 ani mals. The relative frequency of the A allele was 0.52; B allele 0.48; the level of homozygosity 0.501; relative frequency of the AA genotype was 0.24; AB 0.55; BE 0.2. The theoretical frequencies of the genotyp es were not significantly different compared to real ones. From the ci tations of the relevant literature it is obvious that allele A is prev alent, mainly in Holstein-Friesian cattle.