HETERODUPLEX GENERATOR IN ANALYSIS OF RH BLOOD-GROUP ALLELES

We describe the use of heteroduplex analysis to enhance the resolution of different rhesus-derived (Rh) isotypes, Heteroduplex analysis of d ifferent domains of the Rh D and Rh CE loci can be performed to diagno se a variety of blood group incompatibilities. One application of this technique is the ability to test for fetal-maternal blood group incom patibilities during pregnancy, Several new serotype-specific sequence variations were discovered in the mapping of the Rh locus, and used in the construction of artificial heteroduplex generators (HGs). HGs fac ilitate the resolution of the Rh isotypes by electrophoretic methods.