A NOVEL, EFFICIENT REGENERATING METHOD OF NADPH USING A NEW FORMATE DEHYDROGENASE

Authors
SEELBACH K RIEBEL B HUMMEL W KULA MR TISHKOV VI EGOROV AM WANDREY C KRAGL U
Citation
K. Seelbach et al., A NOVEL, EFFICIENT REGENERATING METHOD OF NADPH USING A NEW FORMATE DEHYDROGENASE, Tetrahedron letters, 37(9), 1996, pp. 1377-1380
Citations number
25
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
37
Issue
9
Year of publication
1996
Pages
1377 - 1380
Database
ISI
SICI code
0040-4039(1996)37:9<1377:ANERMO>2.0.ZU;2-Q
Abstract
A NADPH regenerating system using a new, protein engineered formate de hydrogenase (FDH) is investigated. The new enzyme is the first known N AD(P)H dependent FDH. It can be successfully employed in synthesis vii th other enzymes requiring a NADPH regeneration. All advantages of the known NAD(H) dependent FDH's in these enzyme coupled synthesis can no w be transferred to NADP(H) dependent systems.