MUTATIONS IN THE ANOPHELES-GAMBIAE PINK-EYE AND WHITE GENES DEFINE DISTINCT, TIGHTLY LINKED EYE-COLOR LOCI

New eye-color mutations were induced in the mosquito Anopheles gambiae by EMS or gamma-irradiation treatments, Seven new sex-linked mutation s were isolated, five of which were viable and fully fertile, Of those , three were in the previously described pink-eye (p) gene in which tw o spontaneous mutations have previously been identified, Two other mut ations, w(1) and w(2), were in a gene with no extant mutant alleles th at we designate the white gene, One of these, w(1), is due to a large deletion in the 5' end of the cloned homolog of the D. melanogaster wh ite gene, The pink-eye and white loci are tightly linked with recombin ation frequencies of 3.5% and 1.1% between w(1) or w(2) and the sponta neous mutant allele, p(w), respectively, Small samples of F-2 larvae w ere examined for intragenic recombination between various alleles, but none was observed in any experiment, The white mutants, but not the p ink-eye, exhibit epistasis over the expression of the larval body pigm entation phenotype collarless(+) and pigmentation of the male accessor y glands and testis sheath, These pleiotropic effects are similar to t hose of D. melanogaster white mutants and also suggest that white is p robably identical to the previously described white-eye gene.