3 REGION-SPECIFIC MICRODISSECTION LIBRARIES FOR THE LONG ARM OF HUMAN-CHROMOSOME-2, REGIONS Q33-Q35, Q31-Q32, AND Q23-Q24

Three region-specific libraries have been constructed from the long ar m of human chromosome 2, including regions 2q33-35 (2Q2 library), 2q31 -32 (2Q3) and 2q23-24 (2Q4). Chromosome microdissection and the MboI l inker-adaptor microcloning techniques were used in constructing these libraries. The libraries comprised hundreds of thousands of microclone s in each library. Approximately half of the microclones in rite libra ry contained unique or low-copy number sequence inserts. The insert si zes ranged between 50 and 800 bp, with a mean of 130-190 bp. Southern blot analysis of individual unique sequence microclones showed that 70 -94% of the microclones were derived from the dissected region. 31 uni que sequence microclones from the 2Q2 library, 31 from 2Q3 and 30 from 2Q4, were analyzed for insert sizes, the hybridizing genomic HindIII fragment sizes, and cross-hybridization to rodent species. These libra ries and the short insert microclones derived from the libraries shoul d be useful for high resolution physical mapping, sequence-ready reage nts for large scale genomic sequencing, and positional cloning of dise ase-related genes assigned to these regions, e.g. the recessive famili al amyotrophic lateral sclerosis assigned to 2q33-q35, and a type I di abetes susceptibility gene to 2q31-q33.