LIQUID-LIQUID REVERSED MICELLAR EXTRACTION FOR ISOLATING ENZYMES - STUDIES ON THE PURIFICATION OF ALPHA-AMYLASE

Authors
CHANG QL CHEN JY
Citation
Ql. Chang et Jy. Chen, LIQUID-LIQUID REVERSED MICELLAR EXTRACTION FOR ISOLATING ENZYMES - STUDIES ON THE PURIFICATION OF ALPHA-AMYLASE, Process biochemistry, 31(4), 1996, pp. 371-375
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
31
Issue
4
Year of publication
1996
Pages
371 - 375
Database
ISI
SICI code
1359-5113(1996)31:4<371:LRMEFI>2.0.ZU;2-#
Abstract
The purification of alpha-amylase from a elude enzyme preparation was carried out by liquid-liquid reversed micellar extraction. After a ful l forward and background extraction cycle, the alpha-amylase was purif ied about 1.5-fold with 85% recovery. Most of the contaminating neutra l protease in the crude enzyme preparation could be removed at the end of an extraction cycle. The separation factor of alpha-amylase to neu tral protease reached about 10.