DETECTION OF RUBELLA VIRUS-SPECIFIC IMMUNOGLOBULIN-M ANTIBODIES WITH A BACULOVIRUS-EXPRESSED E1 PROTEIN

Authors
SCHMIDT M LINDQVIST C SALMI A OKERBLOM C
Citation
M. Schmidt et al., DETECTION OF RUBELLA VIRUS-SPECIFIC IMMUNOGLOBULIN-M ANTIBODIES WITH A BACULOVIRUS-EXPRESSED E1 PROTEIN, Clinical and diagnostic laboratory immunology, 3(2), 1996, pp. 216-218
Citations number
44
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
Journal title
Clinical and diagnostic laboratory immunology → ACNP
ISSN journal
1071412X
Volume
3
Issue
2
Year of publication
1996
Pages
216 - 218
Database
ISI
SICI code
1071-412X(1996)3:2<216:DORVIA>2.0.ZU;2-R
Abstract
The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombin ant E1 envelope glycoprotein was purified by immunoaffinity chromatogr aphy and used to detect RV-specific immunoglobulin M antibodies in a t ime-resolved fluoroimmunoassay. Correlation analysis between the react ivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV ant ibodies of the immunoglobulin M type.