Three-phase fluidized bed bioreactors have advantages over conventiona
l chemical reaction systems. There is a lack of agreement over most ma
jor operational conditions, and a wide range of design variables are o
pen to question. A large body of recent work in the field has been rev
iewed, with a degree of historical comparison and discussion. It has b
een found that aspects of fluidized bed biofilm reactors of vital impo
rtance include: choice of solid media, gas and liquid loadings, bacter
ial type and reactor mechanical design. A large proportion of the work
in the field of three-phase fluidization is non-biologically specific
, or not tested on a bacterially inoculated system. The majority of th
ree-phase fluidized bed bioreactor work is in the field of water treat
ment. Although this work has highlighted the potential for use of bio-
fluidized beds for this application, there are still specific problems
hindering the large scale industrial acceptance of three-phase fluidi
zed bed bioreactors.