Biochemical studies indicate that the Drosophila timeless protein (Tim
) is a stoichiometric partner of the period protein (Per) in fly head
extracts. A Per-Tim heterodimeric complex explains the reciprocal auto
regulation of the proteins on transcription. The complex is under cloc
k control, and many circadian features of the Tim cycle resemble those
of the Per cycle. However, Tim is rapidly degraded in the early morni
ng or in response to light, releasing Per from the complex. The Per-Ti
m complex is a functional unit of the Drosophila circadian clock, and
Tim degradation may be the initial response of the clock to light.