SIMILARITIES BETWEEN INITIATION OF V(D)J RECOMBINATION AND RETROVIRALINTEGRATION

In the first step of V(D)J recombination, the RAG1 and RAG2 proteins c leave DNA between a signal sequence and the adjacent coding sequence, generating a blunt signal end and a coding end with a closed hairpin s tructure. These hairpins are intermediates leading to the formation of assembled antigen receptor genes. It is shown here that the hairpins are formed by a chemical mechanism of direct trans-esterification, ver y similar to the early steps of transpositional recombination and retr oviral integration. A minor variation in the reaction is sufficient to divert the process from transposition to hairpin formation.