POLYMORPHISM AND CHROMOSOMAL LOCALIZATION OF THE GI-FORM OF HUMAN GLUTATHIONE-PEROXIDASE (GPX2) ON 14Q24.1 BY IN-SITU HYBRIDIZATION

We have isolated a 3.3-kb DNA containing the two exons and a 2.6-kb in tron of the human GPX2 gene, This gene encodes the intestinal isoenzym e of glutathione peroxidase, GSHPx-GI, Consistent signals were detecte d at 14q24.1 when this DNA was used as a probe for fluorescence in sit u hybridization on metaphase chromosomes, Based on either single-stran ded conformation polymorphism or sequencing analysis, two sites contai ning DNA polymorphism were found in the intron: the 5' end had a singl e A/T alteration, and the 3' end had a microsatellite of TC repeats. ( C) 1996 Academic Press, Inc.