AB-INITIO INVESTIGATION OF THE MOLECULAR-STRUCTURE OF METHYL METHOXYMETHYL PHOSPHONATE, A PROMISING NUCLEASE-RESISTANT ALTERNATIVE OF THE PHOSPHODIESTER LINKAGE
M. Strajbl et J. Florian, AB-INITIO INVESTIGATION OF THE MOLECULAR-STRUCTURE OF METHYL METHOXYMETHYL PHOSPHONATE, A PROMISING NUCLEASE-RESISTANT ALTERNATIVE OF THE PHOSPHODIESTER LINKAGE, Journal of biomolecular structure & dynamics, 13(4), 1996, pp. 687-694
Conformational flexibility of the methyl methoxymethyl phosphonate ani
on (CH3-O-PO2-CH2-O-CH3)(-), a nuclease resistant alternative to the p
hosphodiester linkage in DNA, have been investigated by ab initio quan
tum mechanical calculations. The potential of backbone torsional degre
es of freedom of methyl methoxymethyl phosphonate anion (MMP) was dete
rmined at the Hartree-Fock (HF) 3-21G level using the adiabatic mappi
ng technique. Energies, geometries, and effective atomic charges of di
fferent conformers were calculated at HF/6-31G and MP2/6-31G* levels
of theory. These were compared to the results obtained for dimethyl ph
osphate calculated at the same level. The impact on DNA structure from
inserting a methylene group between phosphorus and oxygen of the nucl
eoside sugar moiety was examined via distance- and angle-constrained g
eometry optimizations. Due to its high flexibility, MMP has been shown
to be compatible with both A and B forms of DNA.